Pearson Education Company and Its External Factors Essay

Pearson Education Company and Its External Factors - Essay Example Like any other organization, Pearson Education does not exist in a vacuum but operates in an environment that is characterized by stiff competition from other rival competitors like McGraw-Hill, Reed Elserivier, and Thomson among others. In this context, STEEPLE analysis mainly takes into consideration the environmental factors that may impact on the operations of business. According to information obtained online, there are mainly seven factors that affect the operations of business and these are social, technological, economic, environmental, political, legal and ethical. (See appendix 1). It is very important for any business to diagnose the external environment in which it is operating in order to gain a competitive advantage over the other competitors. An organization ought to strategically streamline its operations so as to be able to remain viable and profitable while at the same time ensuring that its operations conform to the expectations of the customers. Whilst there are v arious external factors, technological as well as economic factors form the basis of this study as going to be analyzed in detail. On the other hand, a SWOT analysis defines the relationship between the internal and external environmental factors in the appraisals in strategic analysis of the operations of a business (Robinson 1997). Basically, SWOT stands for strengths, weaknesses which represent the internal environment of the firm while opportunities and threats are external environmental factors. It is primarily concerned with analyzing the key factors of the environment and the fundamental internal strengths and weaknesses of the organization which will help dictate the strategies appropriate to the firm. The internal factors include the operations of the employees at Pearson Education while the external factors are mainly concerned with technological developments as well as competition from the other rival players.

Plaths Suicide: Considering Lazarus

Plaths Suicide: Considering Lazarus Born on October 27, 1932, in Boston, Sylvia Plath mainly wrote confessional poetry. Plaths life was marked by periods of severe depression and multiple suicide attempts. The death of her father and betrayal of her husband forced her into a state of paranoia and motivated her to write poems about her sufferings. In the poem, Lady Lazarus, Plath employs the character of Lady Lazarus to echo the poets self as a way of expressing her mania towards death and suicide. The character of Lady Lazarus attempts to commit suicide every decade. However, each time Herr Doctor revives her and portrays her like the biblical Lazarus and a walking miracle. Through out the poem Lady Lazarus struggles to regain control over her proclaimed art of dying and becomes stronger as the poem progresses. Plath uses figurative language, most specifically allusions to both the bible and to the holocaust, as well as metaphors of her mental illness and instability to illustrate her growing obsession with death and f oreshadow her third attempt at suicide. Ever since her first attempt at suicide in 1953, Plath has displayed a â€Å"long standing† interest in the biblical story of Lazarus. In this poem, Plath uses allusion to the biblical story of Johns Lazarus of Bethany to juxtapose the character of Lady Lazarus with the biblical Lazarus and character of Herr Doktor with Jesus. In the story of Lazarus of Bethany, Jesus astonishingly raises Lazarus from the dead. Similarly, Lady Lazarus is revived by Herr Doktor from each subsequent suicide attempt. However, when Jesus raised Lazarus from dead, not only was it to advertise Gods power but it was also beneficial to both Lazarus and the spectators who were given hope of immortal life. In contrast, when Herr Doktor revives Lady Lazarus he is interfering with Lady Lazaruss proclaimed art of dying. Herr Doktor is actually establishing dominance over her which she must fight to regain control over her life. He is creating power struggle between them which leads to Lady Lazaruss obsessi on with death and suicide. As Theresa Collins points out, Lady Lazarus can be interpreted as a struggle for control [. . .] a dominion prevented by her torturer, Herr Doktor. In addition, Herr Doktor revives Lady Lazarus in front of a crowd and portrays her as â€Å"A sort of walking miracle†¦Ã¢â‚¬  because similar to Jesus he is longing for admiration and acknowledgement from the people whom Plath refers to as â€Å"The peanut-crunching crowd.† Plath uses irony throughout the poem but her most obvious irony is in her proclaimed art of dying. Plath mentions, â€Å"Dying Is an art, like everything else, I do it exceptionally well.† This quote is ironic because even though Plath brags about being talented at committing suicide she always survives. Every ten years she attempts to commit suicide but each time she is revived by the doctors. Plath is thwarted on each attempt by her torturer, Herr Doktor. It is ironic to see that Plath actually does not want to die on her first time because she states that â€Å"I am only thirty. And like the cat I have nine times to die†. On one hand Plath is struggling to regain control over her art and on the other hand she accepts the fact that she will not be able to regain control until her ninth suicide attempt and has actually stopped trying. Plath feels that she is only living to entertain the crowd by miraculously surviving each time. 1 para about the tone of the poem. 3 paras about holocaust 3 paras about life mental disorder and reference Geopolitics of Food | Article Analysis Geopolitics of Food | Article Analysis Hans Goder In a world, which constantly needs more and more food and facing also some ecologic issues, a change in our way of producing and managing the outputs is needed to face the future challenges (population of 2050). As it is a global challenge, it is the role of the international bodies such as the WTO or the FAO to find and implement those changes. But the question is, are those bodies still have the means to response to this challenge. 2. Primary Sources This academic article written by Bezuneh, Mesfin, Yiheyis and Zelealem for the University of Atlanta, published in the Journal of Economic Development in 2014and supported by many other studies related to the same subject, try to see if trades liberalization has a positive effect on the food security in developing country. As 98% of the undernourished persons of the world are in the developing countries where the average of undernourished is around 15% (FAO, 2014), the present article wants to understand the possible correlation (which can be either positive or negative) between food security in countries which are opening their trades (through regulations and agreements) and introducing themselves in the global market. According to this article, food security is not just the amount of food present in the country. It is reach when the population at all-time have the physical, economic and social access to sufficient, nontoxic and nutritious nutriment which encounters their dietary needs for a healthy and proper life (Bezuneh, Mesfin, Yiheyis and Zelealem, 2014) Methodology used: The methodology used in this article is clear and can be compared to the scientific way of studying a subject. The authors chose 37 different developing countries in different regions of the world. Those countries at the time were encountering the opening of their trades to the world. Then, they compared the figures of the food security (based on the per capita daily energy supply (DES)) before and after the trades opening hoping that a clear pattern and correlation would be seen amongst the results. Also, the authors used some other characteristics to see and understand which of the different factors have the most important effect on food security. Those characteristic were for instance, the political stability of the country and time’s evolution. Then they put those characteristics into figures and ratios, in a mathematical and statistical function to compare each characteristics and its effect on the food safety compared to the trades liberalization factor. Then the result and the patterns were represented into chart and table to improve the understanding of the results. As stated, the overall methodology used in this article was the scientific one following the hypothesis, research, study and finally result pattern. Results: The effects of the opening of the market were not as strong as expected. In fact, those effects were slightly positive but not as relevant as we thought. According to the authors, those positive outcomes could have been done by other thing than the trading policies which were not in the study, once those factors were controlled, the outcome came to be, in some cases, even negatives. Again, according to the authors, this result is to be considerate with caution as the factors and so on are difficult to put into figures at this wide range. But at the end of the day, this study sort out that the relationship between food safety and trades liberalization (which is the main objectives of organization such as the world trade organization) could be considerate as weak, which follows the results of previous studies (Stiglitz and Charlton, 2005). Moreover, still according to the study the relationship between the political stability and food security is even stronger. To conclude thi s study shows that the trades’ liberalization is an important factor to help those countries (through economic growth) but it is not to be used on its own. It needs to be completed by other actions and strategies in order to see a real positive outcome at the end. According to another study led by Stiglitz and Charlton in 2005 – 2006 entitle Aid for Trade, the authors, through a structured analysis admit that developed countries, when on the global market war field, benefit from advantages against the developing country. For them, in order for poor county to benefit from the opening of their market they have to benefit from advantages or ‘’aid’’ to counterbalance the equilibrium of positive outcomes for both sides. For the authors, increasing aid is vital for the poor countries if they are to grasp the opportunities provided through trade and meet transition cost (Stiglitz and Charlton, 2006). Moreover, the authors sort out that the adjustment effort in summit such as the Doha round would be too costly for the poor country due to the loss of the preferences that they are benefiting from. Again, as a conclusion, this article state that trade and aid won’t be the great solution for food and development security. It is just one of the multiple factor that can enhance the development of those countries. Both of the articles tend to state that liberalization of trades, which is encouraged by non-governmental bodies to reduce hunger in the world, might not be the solution. Counting just on this factors could arm those economies on a long run basis and worsen the issues. 2. Secondary Sources The article entitled ‘’Trade and Transnational Corporations: the Solution to World Hunger or a Major Part of the Problem?’’ , written by Peter O’Driscoll, expert in the field of developing markets, speaks about the effect of NGO’s such as the World Trade organization and Food and Agriculture Organization of the United Nations on food insecurity in developing countries. The organizations have as main weapon against this plague of food insecurity the liberalization of trades through opening regulations and the insertion of those countries in the global market. According to this article, effectively, during the liberalization era, the amount of international agricultural trade has increased by 800 million tons per year. Through the article, the authors state that this increase has benefited mostly to the developed country and, in the contrary, destroyed and dislocated developing countries’ agricultural industries. Moreover, the article sort out another critical point, the number of hungry persons in the world between 90 ( pre-liberalized era) and 2002 (post liberalization) has increase by a huge amount around 18 million people. In addition, subsidiaries and economic dependence have worsened the situation. While country such as India use those subsidiaries to feed its population by decreasing prices, it affect the world worldwide as other farmers around the world need to decrease their price in order to be competitive. This reduces their turnover, which can at term led to a huge crisis. De Schutter,O in his study ‘Droit à   l’alimentation : une question de qualità ©, non de quantità ©Ã¢â‚¬â„¢ speaks about the food fundamental right and state that the base of the problem could come from an inequality in the food distribution between the developed countries and the developing countries. To improve this situation, the author doesn’t put the liberalization of trades as the solution. In the contrary De Schutter state that the solution would be to change between a quantitative focused industries to a qualitative based one where the food question will be put as first priority and the economic aspect as the second. For him, doing that won’t reduce the turnover of organization; contrarily it will open new market and provide new opportunities for agriculture based organizations. Moreover, the writer also states that it would be more effective to concentrate resources into improving their own agriculture sector instead of increasing their dependence to the global market. According to Chris Arsenault in his article ‘’Global dependency on food imports makes countries vulnerable’’, the global prices are at their lowest level in five years. But still according to the author, due to population increment and also standards of living improvements, those low prices won’t last long. When those prices will start to go up, the developing countries which are vulnerable and dependent to the global economy will have to face a disastrous crisis. To prevent this, the authors state that government in those countries should be smart and protect their arable earth and try to reduce their dependence on food import. Finally, according to this article, the market has an important role to play but it shouldnt be the final arbiter of who gets food and where it comes from (Arsenault, 2015). The report entitled The State of Food Insecurity in the World of the FAO in 2010 shows us that some part of the non-governmental organization has already understood the importance of the qualitative point of view. In fact, the improvement of the support to livelihoods within the country could be a better solution than trades itself but an re-architecture should be apply for it to be more effective and obtain some long term benefits. This would increase the strength of the country, it development, it stability and so one but a better understanding is required. Moreover, this article do state that doing that would be benefic for the developed country, as those stable country would become interesting markets full of opportunities on the long run. 3. Etat de l’art Those works and articles present in the previous parts of this state of the art represent a brief surrounding of all the ideas and studies present nowadays in this field of study. To sum up, they all tend to say and prove in different way and through different methodology and approach, which can be scientific or not, that trades liberalization and by extension globalization couldn’t and can’t be a good response to food insecurity in poor and developing countries around the world on its own. According to some of those articles, in some cases this facilitation and liberalization could be armful for those weak and dependent economies. As we already know, the fight against food insecurity is led by some non-governmental organization such as the World Trade Organization, the Food and Agriculture Organization of the United Nation or the World Bank. Those organization, first have their headquarters in developed country such as the United States, where trades, profits and wealth are the priorities. Secondly, they might have a wrong strategy to win this plague. Indeed, those NGOs, since the beginning of this combat, want to answer it through an economic and trade based point of view, which, for De Schutter could be qualified as the quantitatives optics (De Schutter O. 2013) through summit such as the Doha cycle and the Bali one. Concretely, they try in most of the cases to make the developing countries open its barriers and incorporate the global market. In addition, they help those countries to develop their own organization in order, at the end, to make them trade on this global market. But the problem is that, according to many studies, this strategy tends to be more profitable for the developed country (O’Driscoll P, 2014) as they are provide with new markets where competition is easy to handle. For the developing countries, the findings are more mitigated. When they try to enter this huge market, where competition is much bigger and hard, they are obliged to compete by reducing the prices and by doing that they can’t really develop themselves. Moreover, after this opening, those weak countries become highly dependent and vulnerable to the international market. Through that, when some distortions happened within the global economy through the market rules, it strikes even more those countries as their populations are more sensible to price fluctuation and also as their economy is weaker than the developed ones. For example, between 2006 and 2009 the number of undernourished people has increase due to price fluctuations (FAO, 2010) These statements should be taken with precautions. In fact, according to some other articles, the correlation between food insecurity and trades opening is not really clear and strong but this factor stays a key factor for life quality and improvement through economic growth (Stiglitz and Charlton, 2005). Moreover, trades facilitation might be a short-run solution but not a long-run one. What we have to understand is that we can’t just rely of this only factor to be able to reach the objective of 2050 which is to feed 9 billion people. Taking and understanding all those ideas we will use and go further in those study by using and applying them to the core NGOs, and see through a quantitative and qualitative research method if whether or not the existing non-governmental organization are still able to provide effective strategies and solutions to the upcoming challenges through their present point of view or if a rethinking of their entire goals and approaches is needed to provide long terms solutions. To do so, we will structure our thoughts in a specific way. Initially, we will have a deeper look at diverse other information related to the same field and problematic stated earlier to gatherer some crucial inputs. Then, we are going to have a close look to the different bodies present in the world according to the angle chosen and their different actions and outcomes. Finally, founded on the previous assumptions, we will try to sort out the opportunities of the situation and give also, if case is, some concrete solutions and strategies that might improve the present situation. References De Schutter, O. (2013). Droit à   l’alimentation : une question de qualità ©, non de quantità ©. [online] Opinion-internationale.com. Available at: http://www.opinion-internationale.com/2013/11/28/droit-a-lalimentation-une-question-de-qualite-non-de-quantite_20844.html [Accessed 2 Dec. 2014]. Arsenault, C. (2015). Global dependence on food imports leaves countries vulnerable. [online] Reuters. Available at: http://www.reuters.com/article/2015/03/13/us-food-trade-idUSKBN0M92CG20150313 [Accessed 1 May 2015]. ODriscoll, P. (2014). World Hunger Notes Trade and Transnational Corporations: the Solution to World Hunger or a Major Part of the Problem? Peter ODriscoll. [online] Worldhunger.org. Available at: http://www.worldhunger.org/articles/05/global/odriscoll.htm [Accessed 1 May 2015]. Stiglitz, J. and Charlton, A. (2006). Aid for Trade. 1st ed. Commonwealth Secretariat. FAO, (2015). The State of Food Insecurity in the World. 1st ed. FAO. Shah, A. (2015). Foreign Aid for Development Assistance — Global Issues. [online] Globalissues.org. Available at: http://www.globalissues.org/article/35/foreign-aid-development-assistance [Accessed 1 May 2015]. Kumar, R. and Nair, S. (2009). INDIA: STRATEGIES AT THE DOHA DEVELOPMENT AGENDA- JULY AND BEYOND. Geneva, p.4. lOMC, (2013). OMC | Nouvelles 2013 Jours 3, 4 et 5: Un â€Å"Paquet de Bali† voit le jour à   l’issue de consultations-marathon. [online] Wto.org. Available at: https://www.wto.org/french/news_f/news13_f/mc9sum_07dec13_f.htm [Accessed 4 Mar. 2015]. Cho, S. (2006). The WTO Doha Round Negotiation: Suspended Indefinitely | ASIL. [online] Asil.org. Available at: http://www.asil.org/insights/volume/10/issue/22/wto-doha-round-negotiation-suspended-indefinitely [Accessed 2 Mar. 2015]. FAO, (2009). FAO Nouvelles: 2050: 2,3 millliards de bouches de plus à   nourrir. [online] Fao.org. Available at: http://www.fao.org/news/story/fr/item/35656/icode/ [Accessed 10 Mar. 2015].

Macbeth: Letter From Lady Macbeth To Macbeth :: Macbeth essays

Macbeth: Letter From Lady Macbeth To Macbeth Dearest husband, These last few months have been sorry ones. The actions you and I have undertaken have played on my conscience, and I am finding it hard to cope. I realise that the path we had chosen was the wrong one, and even though I accepted that from the beginning, the consequences of that choice and the mental anguish that I am experiencing now were unimaginable at the time. I was pleased when I received your letter telling me of your promotion to Thane of Cawdor. I am sure it was a step that pleased you too. But I'm afraid that my ambition to act on the witches prophecies was to be our downfall. The prospect of you being king was so great that I lost touch with reason. When the idea of murdering the king was put forth I know you were hesitant on acting, but I just had to have my ambition fulfilled. So I pushed you and now I realise that my persistence was not in our best interests, and I'm sorry for it. Perhaps if I had left the decision to your judgement we would have been better off. What I did I only did for you. For you to be king, how could I for see that we would be worse off. Just the idea that you would be king "would cheer me ever", but I knew I had to push you to fulfill your potential. But doubt crept into my mind on the fateful night of Duncans murder. I would have done it myself if he had not looked like my father. He was resting so peacefully in the innocence of sleep, I just couldn't bring myself to do it. So you had to. Who could have imagined the old man would have had so much blood in him. This blood has stained me forever and I am afraid it has done the same to you. Nothing can remove this blood. Many nights I would wake in cold sweat and my hand would be red from my rubbing. The blood just wont leave me, it haunts me night and day. But the murder of the Macduff family was just too much. Sometimes I can hear the cries of the women and young children as they are being killed, and it is the knowledge that my decisions led to their deaths that I find most distressing. It is ironic that the actions leading to my demise have also led to

A manager and a leader Essay

The difference between a manager and a leader is that the former ensures control and rationality, her/his focus is on day to day problem solving, best means in achieving results for staff to continue to contribute to the organisation (Zaleznik,1992) . While a leader uses power to influence actions and people, focusing more on outcomes and impact, where this action has inherited risks such as the risk of losing self- control in the need for power (Zaleznik,1992). While leaders and managers have common traits such as sound communication skills, work ethics, integrity, key technical competencies, etc, yet there are some differences. These differences can be summarised as follows: Risk taking: Manager are regulators of affairs in an organisation, getting rewards from collective achievements . Therefore, to be a good manager, it requires persistence, patience, intelligence, analytical skills, and goodwill. Leaders think out of a box and work with high risk initiatives. While managers, unlike leaders, tend to solve problems in a more conservative way and tend to tolerate mundane and practical work (Zaeznik,1992). Leaders on the other hand are risk takers. Relationships: Manager work with people to implement initiatives, solve problems, by focusing staff on policies and procedures and not on content (Zaleznik,1992). They are engrossed in how to make decisions, but not what are the rights decisions to make. While leaders attract strong feelings of identity and difference. Their human relationship might seem turbulent, intensifying motivation and often resulting in unanticipated results (Zaleznik, 1992). Sense of self-worth: managers’ sense of self-worth is secured by ensuring and strengthening existing institutions. While leaders feel separate from their institutions, their perception of identity is different (Zaleznik, 1992). They may work in an organisation and never have a sense of belonging, always seeking opportunities for change. Vision versus objectives and goals: Managers take the short view, focus on objectives, targets, implementation and monitoring and evaluation while leaders take the long view, focusing more on a clear vision, a clear sense of purpose, and a passion to pursue that vision while aligning the organisation its vision (Allio, 2012). Manager Leader Ensures control and follows day to day work implementation of an action plan (day to day work) Sets a vision A problem solver Uses power to lead and influence action Not a risk taker Willing to take risks (balance between change and stability) Follows rules and regulations High levels of creativity, always thinks out of a box Implementer Leads, inspires and motivates Strong sense of belonging to organisation May and may not have a sense of belonging to organisation Works in a comfort zone Always embracing change management, seeking new opportunities. In health care, the Minister of Health in the State of Palestine is a leader, while the director generals, directors of various departments are managers. The former is the person who sets out the vision for the ministry, partnering with other stakeholders (UN organisations, private sector, and others) for instance in advancing the public health sector. This resulted in a reduction in infant and under five child mortality rates over the past five years. While director generals and directors are consumed with implementing the inputs needed to achieve the overall vision set out by the Minister. Thank you. References: Allio, R.J., (2012), ‘Leaders and leadership – many theories, but what advice is reliable?.’ Strategy and Leadership. [Online], 41, (1), pp. 4-14. Available online from: http://www.emeraldinsight.com.ezproxy.liv.ac.uk/doi/pdfplus/10.1108/10878571311290016 (Accessed on: 19 January 2015). Zaleznik, A., (1992), Managers and leaders: Are they different?’ Harvard Business Review. [Online], 70 (2), pp 126-135. Available from: http://eds.a.ebscohost.com.ezproxy.liv.ac.uk/eds/pdfviewer/pdfviewer?vid=3&sid=ef29e84c-a001-4d56-9f66-b5f4a202c402%40sessionmgr4001&hid=4202 (Accessed

Garden Report Essay

The Garden Depot, a family-owned floral company, carries a larger variety of floral, gardening and lawn-care products with sales and profits growing. Janice bowman has 13 years of operational knowledge in the industry as office manager in the company. She is responsible for inventory management, computer system management and logistics. Derek Sinclair, the son-in-law of the Depot owner, is the landscaping manager with no experience in management. However, the operation is not smooth in the organization that faced several issues such as lobar force, communication and synchronization. They both have responsibility for the depot, and have to get rid of problems to benefit the company. The company did not have enough permanent skillful and reliable workforce, but only seasonal employees, therefore 50 landscaping jobs could be completed each year, which were fewer than what was demanded. Most of employees were part-time student workers lacking of experience. In addition, a family member who had poor and inappropriate leadership was appointed as landscaping manager. And his irresponsible attitude might have bad impact on moral of stuff. Furthermore, the job responsibilities were not defined, and there were no specialized department to solve the specific problems. The improper recruitment and lack of supervision cause the organization attained unstable and unreliable workforce. This demonstrates a lack of fit between organization process and people as police for recruiting and supervising employees. Moreover, Sinclair had low expertise knowledge on landscaping area, which resulted that he could not answer the customers’ specific question, and he had no qualification and business handling capabilities to solve complex issue. This follows EOPT theory because there is a lack of fit between people and tasks. Whereas having no job description and departmentalization in the organization resulted in the consequence that employees confused about their job. This applies to EOPT theory because there is a lack of fit between organization structure and people. The goal for the Garden Depot is to establish stable and reliable workforces to increase work proficiency. In long term, the organization should ensure job responsibilities defined in every level of the organization, and deploy individual departments to solve any problems to make customers happy. The first alternative is to reevaluate all the stuff and build a formal appraisal system to supervise employee’s performances, which can help the company to build a stable and reliable workforce. The second alternative will be training Sinclair be a competent manager. If the company wants to keep Sinclair as the landscaping manager, this action alternative is necessary. The last alternative course of action is to create a proper job description and deploy specific departments such as HR department, marketing department, customers departments and inventory departments in order to solve the related issues. The best alternative is to create a formal job description and divide specific departments. First, Sampson should divide the whole organization into four departments HR department, marketing department, inventory department and customer services department. Next, creating job description for every position should be done as soon as possible. Then department supervisors ought to reevaluate employees and put them in the right departments depending on their specialty. Finally, the department supervisor should hand in a process report to managers every month to show their work. If Departmentalization causes lack of communication among individual departments, general manager should have a meeting with all department supervisors once a week.

Measuring Things In Various Sports - ESL Quiz

Measuring Things In Various Sports - ESL Quiz This is a series of two quizzes focusing on sports vocabulary. The first quiz deals with measuring sports, and the second quiz on sporting venues. Time, the score and distance are measured in various ways depending on which type of sport you are speaking about. Decide which time, score and/or distance measurement is used in each of the sports below. Some of the words are used more than once: game, point, set, mile, inning, strokes, yard, round, move, match, meter, round, quarter, out, half, lap, down, length American Football: _____European Football: _____Tennis: _____Chess: _____Swimming: _____Ping Pong: _____Horse Racing: _____Ice Hockey: _____Boxing: _____Volleyball: _____Athletics: _____Motor Racing: _____Baseball: _____Racketball: _____Squash: _____Golf: _____    Here are the answers to the previous quiz: American Football: point, down, quarter, half, yardEuropean Football: point, meter, halfTennis: point, game, set, matchChess: move, gameSwimming: length, meterPing Pong: point, gameHorse Racing: lap, lengthIce Hockey: point, quarter, half, gameBoxing: roundVolleyball: point, gameAthletics: meter, yardMotor Racing: lap, miles, metersBaseball: point, inning, outRacketball: point, gameSquash: point, gameGolf: stroke    The question above could be answered with pitch or field depending on whether you are talking about European football or American football. Sports take place on/in all sorts of different areas. Decide whether the sport is played on/in the following areas. Some of the words are used more than once: court, rink, table, course, field, ring, pitch, board, track, ring, field, pool American Football: _____European Football: _____Tennis: _____Chess: _____Swimming: _____Ping Pong: _____Horse Racing: _____Ice Hockey: _____Boxing: _____Volleyball: _____Athletics: _____Motor Racing: _____Cricket: _____Baseball: _____Racketball: _____Squash: _____Ice Skating: _____Golf: _____    Here are the answers to the previous quiz: American Football: Field European Football: Pitch Tennis: Court Chess: Board Swimming: Pool Ping Pong: Table Horse Racing: Track Ice Hockey: Rink Boxing: Ring Volleyball: Court Athletics: Track Motor Racing: Track Cricket: Pitch Baseball: Field Racketball: Court Squash: Court Ice Skating: Rink Golf: Course Two More Sporting Vocabulary Quizzes Continue improving your sports vocabulary by taking these two quizzes on proper verb use and sporting equipment.

Cmo se certifica una traduccin para Inmigracin

Cmo se certifica una traduccin para Inmigracin La documentacià ³n en espaà ±ol para Inmigracià ³n debe traducirse  al inglà ©s. Puede ser  suficiente la traduccià ³n certificada  hecha por una persona bilingà ¼e.   Hay que tener en cuenta que en ocasiones cierto tipo de documentos, como calificaciones acadà ©micas o laborales para su convalidacià ³n, puede que tengan que ser traducidos por un intà ©rprete jurado. Incluso puede ser necesario obtener la Apostilla de la Haya. Pero para la mayorà ­a de los documentos, principalmente en asuntos migratorios  no ser necesario. Y es suficiente con una traduccià ³n certificada hecha por cualquier persona bilingà ¼e por lo que se puede ahorrar dinero en este trmite. Adems, NO es necesaria la firma de un notario. Quià ©n puede hacer una traduccià ³n certificada y cà ³mo se debe realizar Cualquier persona que es amiga del solicitante de un beneficio migratorio (no un familiar) con conocimientos fluidos de los idiomas espaà ±ol e inglà ©s.Debe traducir palabra por palabra. Es decir, no basta un resumen del texto que traduce sino que ha de ser una traduccià ³n fiel y completa.La traduccià ³n debe hacerse en una hoja en blanco, escribiendo con letra legible y tinta negra o -preferible- en computadora. Al finalizar la traduccià ³n es cuando se inserta, en la misma hoja, el siguiente modelo de carta que se seà ±ala a continuacià ³n y en el que se certifica que la persona que la ha traducido tiene conocimientos fluidos de inglà ©s y espaà ±ol y que ha realizado con competencia la traduccià ³n. Ejemplo de modelo de carta que se coloca al final de la traduccià ³n para certificarla I certify that I am competent to translate from Spanish to English and that the above Marriage Certificate of Pedro Sanchez and Marà ­a Garcà ­a is a correct and true translation to the best of my knowledge and belief. Signed: Tito Jandro  Caramà ©sAddress: 163 W. 36th Street, NY NY 10017Phone number: 917  21  63  68Date: February 7th, 2017 Notas sobre esta certificacià ³n de la traduccià ³n Obviamente el nombre o nombres de las personas sobre las que trata el documento y el tipo de documento. En el ejemplo se utiliza un certificado de matrimonio.En Signed, adems de poner el nombre completo de la persona que hizo la traduccià ³n, hay que acordarse siempre de firmar.La direccià ³n y el nà ºmero de telà ©fono han de corresponder a la persona que ha hecho la traduccià ³n. Segundo ejemplo de modelo para certificar una traduccià ³n I, Carmen Pà ©rez, certify that I am fluent in the English and Spanish language and that the above document is an accurate translation of the document entitle Birth Certificate. SignatureName: Carmen Pà ©rezAddress: 163 W. 64th Street NY NY 10017Date: February 7th, 2017 Que junto con la traduccià ³n certificada de esta manera hay que enviar una fotocopia legible del documento original que se ha traducido. Incluir el derecho y el revà ©s. Ejemplos de documentos para los que es suficiente este tipo de traduccià ³n Actas de nacimiento (conocidas en algunos paà ­ses como certificados) que se incluyen en peticiones de familia.Actas de viudedad, divorcio o anulacià ³n de matrimonioCertificado de matrimonioDeclaraciones juradas sobre el buen carcter moral de un migrante, que se recomienda incluir en peticiones como por ejemplo de perdones. Consejos sobre traducciones para Inmigracià ³n Todos los documentos en espaà ±ol que se envà ­an al Servicio de Ciudadanà ­a e Inmigracià ³n (USCIS, por sus siglas en inglà ©s) deben de ser traducidos. Sin embargo, en ocasiones los que se envà ­an a los consulados no siempre es necesario. Va a depender de la polà ­tica de cada consulado, con lo que es conveniente consultar en la pgina web cules son los requisitos. Si se elige  un traductor profesional, deber tambià ©n incluir algà ºn tipo de certificacià ³n sobre la traduccià ³n que realice, por ejemplo siguiendo los ejemplos anteriores seà ±alados en este artà ­culo. Finalmente recordar que no es necesario que estas traducciones està ©n  firmadas por un notario Este es un artà ­culo informativo. No es asesorà ­a legal.

The Benefits of a Post-Graduate Year

The Benefits of a Post-Graduate Year Did you know that each year, a number of high school graduates opt to spend another year at high school? A private high school to be exact, and the enroll in a program known as the post-graduate year, or PG year. Over 150 schools worldwide offer postgraduate programs. Admissions standards vary as do the objectives of the postgraduate programs themselves. It probably makes a certain amount of sense to have a student stay on at his old school for the postgraduate year. If he wants to attend another school, he may find the  admissions process  almost as intimidating as applying to be a first-year student. On the other hand, admissions to a post-grad year at his old school will be a mere formality. Postgraduate years are particularly useful for boys who want an extra year to mature before moving on. The postgraduate year gives young men that little extra confidence which they might lack at the end of 12th grade. A  PG or postgraduate year  is a popular option for many students. Personal Growth/Maturity A post graduate year gives students some extra time to strengthen academic skills, to participate in sports and to prepare for college admissions testing. For many students, it also gives them a little extra time to mature. Not every student is ready for the independent lifestyle at college, nor are they always prepare to live on their own for the first time. A post-graduate year at a boarding school gives students a chance to get used to an independent lifestyle in a supportive and nurturing environment. It can be a great stepping stone to prepare a student for college. Improve College Admissions Chances Many students choose to do a post-graduate year to improve their chances for admission to a particular college. College admissions can be fiercely competitive. If a student has his heart set on getting into a certain college, he may, in fact, be better off waiting out a year in the hopes that his application might be more favorably received. Most private schools offer experienced college counselors to help with the admission process and guide students to craft a personal path to excellence.   Perfect Athletic Skills Other students want to take a year before heading off to college to perfect their athletic skills. From a chance to play on a top team and get noticed by college sports recruiters to strength training and agility preparation, a post graduate year can really give students a leg up on their competition, and get a student noticed by scouts who can get them into top schools. And, many elite athletes earn college scholarships, and a post graduate year can make a student a more desirable candidate.   Schools That Offer a PG Year There is only one school which offers a PG program exclusively. Thats Bridgton Academy in North Bridgton, Maine. All the other schools on the list below offer their PG year as a kind of 13th grade if you will. Here are some schools which offer PG programs: Avon Old Farms School, Avon, CTBlair Academy, Blairstown, NJBrenau Academy, Gainesville, GABrewster Academy, Wolfeboro, NHBridgton Academy, North Bridgton, MECamden Military Academy, Camden, SCCanterbury School, New Milford, CTChapel Hill-Chauncy Hall, Waltham, MACheshire Academy, Cheshire, CTChoate Rosemary Hall, Wallingford, CTChristchurch School, Christchurch, VACulver Academies, Culver, INCushing Academy, Ashburnham, MADarlington School, Rome, GADarrow School, New Lebanon, NYDeerfield Academy, Deerfield, MAEmma Willard School, Troy, NYEthel Walker School, Simsbury, CTFlorida Air Academy, Melbourne, FLFork Union Military Academy, Fork Union, VAThe Forman School, Litchfield, CTFryeburg Academy, Fryeburg, MEGould Academy, Bethel, METhe Gow School, South Wales, NYGrand River Academy, Austinburg, OHGrier School, Tyrone, PAThe Gunnery, Washington, CTHargrave Military Academy, Chatham, VAHebron Academy, Hebron, MEHill School, Pottstown, PAHoosac School, Hoosick, NYHotchkiss School, Lake ville, CT The Hun School of Princeton, Princeton, NJIdyllwild Arts Academy, Idyllwild, CAIndian Springs School, Indian Springs, ALKents Hill School, Kents Hill, MEKimball Union Academy, Meriden, NHThe Kiski School, Saltsburg, PALake Forest Academy, Lake Forest, ILThe Leelanau School, Glen Arbor, MILeysin American School, Leysin, SwitzerlandLinden Hall School, Lititz, PALoomis Chaffee School, Windsor, CTMarine Military Academy, Harlingen, TXMercersburg Academy, Mercersburg, PAMiller School, Charlottesville, VAMissouri Military Academy, Mexico, MOMontverde Academy, Monteverde, FLNew Hampton School, New Hampton, NHNorthfield Mount Hermon School, Northfield, MAOldfields School, Glencoe, MDOrme School, Mayer, AZThe Peddie School, Hightstown, NJPerkiomen School, Pennsburg, PAThe Phelps School, Malvern, PAPhillips Exeter Academy, Exeter, NHPomfret School, Pomfret, CTRandolph-Macon Academy, Front Royal, VASaint Johns Preparatory School, Collegeville, MNSt. Timothys School, Stevenson, MDSouth Kent Scho ol, South Kent, CT Stoneleigh-Burnham School, Greenfield, MASuffield Academy, Suffield, CTThomas Jefferson School, Saint Louis, MOTilton School, Tilton, NHVerde Valley School, Sedona, AZWasatch Academy, Mt. Pleasant, UTWayland Academy, Beaver Dam, WIThe Webb School, Bell Buckle, TNWentworth Military Academy, Lexington, MOWest Nottingham Academy, Colora, MDWestern Reserve Academy, Hudson, OHWestminster School, Simsbury, CTThe White Mountain School, Bethlehem, NHWilbraham and Monson Academy, Wilbraham, MAWilliston Northampton School, Easthampton, MAThe Winchendon School, Winchendon, MAWyoming Seminary Upper School, Kingston, PA Article edited by Stacy Jagodowski

Principles of Economics Essay Example | Topics and Well Written Essays - 750 words - 2

Principles of Economics - Essay Example At ceteris peribus, an increase in the prices of alcohol will cause a fall in the quantity demanded and consumed of a product (Becker, 2001). This would help mitigate the economic impacts of over consumption of alcohol. However, the economists must put into consideration the fact that the demand curve of alcohol is normally inelastic given the fact that it is addictive. They therefore have to realize that an increase in the tax level may lead to a small decline in the quantity of the product consumed. The second option that can be pursued by an economist is moral persuasion. In this case, economists will prefer explaining the marginal cost and marginal benefits of consuming alcohol. In this case, the marginal cost will be higher than the marginal benefits hence economists will promote campaigns against the use of alcohol (Frank & Bernanke, 2007). They will therefore influence the enactment of legislation to bar teenagers from using alcohol; limit the number of drinking hours among ot her measures that reduce the consumption level. When some drugs are prescribed for use, they will influence the demand and supply of other products. For instance, the demand for products used in the manufacture of a drug will increase if the quantity of the drug prescribed has been increased. ... The elasticity of the two curves will determine the effectiveness of the policies formulated to regulate the performance of the economy. A shift in the supply curve will lead to a change in the new equilibrium point. If the demand curve were inelastic, a huge shift in the supply curve would result into relatively small changes in the equilibrium price (Boyes & Melvin, 2008). Elasticity of the demand curve will therefore influence the economic impact of the shift in the supply curve. For instance, a shift in the supply curve would only result in an increase in the equilibrium price if the demand curve were perfectly inelastic. Policymakers will therefore determine the effectiveness of their decisions when considering the steepness of the demand curve (Frank & Bernanke, 2007). Similarly, the elasticity of the supply curve will influence the net effect of a shift in the demand curve. Where the supply is inelastic, a shift in the demand curve will cause little or no change in the equilib rium quantity because even with an increased demand, suppliers will not be able to expand the quantities supplied. Prices will therefore increase but the quantity supplied will remain relatively constant. The elasticity of either the demand and supply curves are normally important when formulating policies aimed at influencing economic performance i.e. the government can increase the taxes imposed on goods with inelastic demand because this would not significantly affect the amount of the good consumed (Becker, 2001). For an increasing cost industry, entry of a new firm will result in an increase in the unit cost of production. When a firm enters the market, it will cause the prices of the resources used in

Management Solutions in Practice Case Study Example | Topics and Well Written Essays - 2250 words

Management Solutions in Practice - Case Study Example With the assistance of the cloud computing, the IT organizations are served with distinct models of operation. Since the cloud providers are experts in particular applications as well as services, they are capable of managing upgrades and maintenances, failover functions, backups and disasters recoveries. Therefore, the customers using the cloud computing services are capable of gaining reliability and there is decline in the cost as well because of economies of scale and other factors of production. The current needs of the organizations can be monitored with the help of cloud computing and therefore they are capable of adjusting the capacities according to their requirements. The standardization of the protocols as well as processes can be stimulated in order to interoperate the many pieces of the cloud computing model (Educause, 2009). Salesforce.com is one of the companies that is offering cloud computing to its customers and is perceived to be one of the disruptive technologies based companies. It has been able to maintain competitive advantage and has made a mark for itself with the help of its innovative business model as well as has achieved resounding success (Salesforce, 2011). The main challenge that the Salesforce will face in the near future is the expansion of the business model in other areas. It has been identified that one of the significant challenges that they will face in the near future is related to increase in competition. The reason behind this is that the industry leaders as well as the new challengers would like to replicate the success of Salesforce. It can be stated that both the globalization and the information technology are collaborating together in order to create an unstable business environment and thus the new entrants as well as the pressing innovations are reducing the income of the

Corporate Social Responsibility Essay Example | Topics and Well Written Essays - 1500 words

Corporate Social Responsibility - Essay Example These can include (but may not be limited to) employees, customers, suppliers, community oraganaizations, local neighborhood and shareholders.'-Wikipedia.CSR entails that corporations should contemplate the actual and impending effects of their decisions on other stakeholders as well. It is correlated with the principles of sustainable development, which propagate that social, environmental and other consequence of business decisions should be taken into consideration. These two aspects can be better understood by examining the shareholders and stakeholders theories. The main ideas of the two theories are juxtaposed under the following heads. Director's fiduciary duties: The Shareholder's theory advocates that value maximization should be the governing corporate objective. It makes a plea that the interest of the shareholders should precede over that of other constituents, based on the presumptions of capitalism. The supporting view is that, the directors fiduciary duty is to run the company in the interest of the shareholders.Tradional property rights are cited as a justification for this argument. The shareholders are the owners of the company, therefore they hold property rights. ... The chief proponents of the stakeholders' theory are Donaldson and Preston. According to this school of thought, directors should have multi fiduciary duties towards all the stakeholders. Stakeholders are the people who affect and are affected by the company. The stakeholders view of strategy is an instrumental theory of the corporatin, integrating both the resource based view and the market based view, it is opposed to the view where the company solely tries to increase the value for shareholding. The stakeholder group should not be treated as a mean to some end but must be participative in detreming the direction of the company in which they hold stake. Basis for determining business relationships: As per the shareholders' theory, business relationships are determined by legal contracts. Thus, it recognizes accountability towards those parties with whom explicit legal contacts have been signed. It follows Sternberg's argument that such contracts are made under 'libertarian free contracting". This vastly limits the scope of the corporation's obligations. On the other hand, the stakeholders' model advocates social and moral obligations towards all those who affect or get affected by the company, these obligations may be become binding on the company via legal or implied contracts. Both the theories accept that the firm is a nexus of contacts but differ about the basis for determing those contacts. Objective of wealth creation: The two theories take different stands about the nature of these contracts. Kay's work in strategic management (Kay1993), show that managing contracts on collaborative, 'relational' basis can lead to competitive advantages directly benefiting shareholders. Thus, shareholder's theory acknowledges that contacts should be

My Experience with the Council Form Essay Example | Topics and Well Written Essays - 1000 words

My Experience with the Council Form - Essay Example The council helped me accept diverse opinions than before, in addition to understanding those who disagreed with me. During the session, I noticed that it was interesting to listen to others particularly those who had different opinions from mine, especially when they supported them with studies and pieces of evidence. My little voice was thinking on their reactions, telling myself that they could be either right or wrong. I did not have a problem listening because I listened to each and everyone in the group and realized that life is cool when we communicate to each other, as well as when we give each other an opportunity to argue and speak his ideas. To add to this, I discovered that, if one believes what he says and have evidence, then your conversation has a great effect on other participants in the group session and everyone will agree with you. I also learned from the controversial topic that we can all discuss and come up with a correct answer, although I did not expect such a topic and therefore, my opinion was not changed since I hate to hear or talk about it, though it was nice to hear people give their opinions on it. During the group meeting and group presentation, some of my strengths as a group participant were supporting my opinions with studies and pieces of evidence, showing that marijuana should be kept illegal. I was also the only member who was against marijuana, and therefore, my position was a specialist opinion. I played the role of trying to convince them in regards to marijuana risks, but at the same time respecting their views, though they insisted that they had to read and see more studies on its risks. However, they tried changing my views but they could not give me any strong and logic pieces of evidence either. I have learned a lot from the group sessions, like having to listen to each and everyone, in addition to, those who disagree with me and respect their opinions. During the preparation of our presentation, leadership emerged from our group, where Tom was elected to be the leader, where he led us effectively. The leadership was also shared, where Eric helped Tom in leading us as well as, helping each one of us. He was helpful as well as a good friend. There was also satisfactory leadership in our group session because we all got A’s. It was also easy for us to make decisions on how our presentation would be structured. This is because we respected and understood each other, which is very important in group work. If group members do not understand and respect each other, the group becomes disorganized in their final presentation. The consensus was easily reached because we all worked as a team although there were members with different views, we still maintained the friendship.  Ã‚  

Naccobus Essay Example | Topics and Well Written Essays - 2000 words

Naccobus - Essay Example The sources will be picked from the list that has been used in the provided study. Each source will be analyzed separately and the reason why it was important to use it will also be given and how it helped in making the paper complete. Sher is this is one of the most important sources that have been used in this study. This source has been used throughout the study to provide concrete information on Nacobbus. Sher gives the required information about the Genus Nacobbus (222-227). According to this source the genus contains only two valid species which are indicated to be the Nacobbus dorsalis and N. aberrans. This source goes ahead to state where these two species are normally found. It indicates that N. aberrans is normally an important parasite of various vegetables like beans, chili, tomatoes and pepper. Further into the study, Sher has been extensively used to give information on variability that has been seen in Nacobbus (222-227). The source has been used to give information on morphological characteristic of N. aberrans. It reveals the various ranges of morphometrics of N. aberrans from various geographical locations in the world, thereby helping in understanding the various characteristics of the organism being studied. The reason why Sher is considered one of the important sources that has been used is the amount of information that has been obtained from this source on the characteristics and morphology of N. aberrans. The second source that has been considered among the 10 most important sources in this study is Thorne (1949). Thorne is talking about the new classification of the Tylenchida. It is one the proceedings of the Helminthological Society of Washington. This source has been used to give information on various discoveries that were made on Nacobbus in different regions of the world. The source has been used to give information on taxonomy of Nacobbus (Thorne 57).

Argumentative Problem - Solution Paper Research

Argumentative Problem - Solution - Research Paper Example Key words: Deforestation, climate change, greenhouse effects and gases, and global warming Introduction The last 100 years have experienced an increase in global temperature by approximately 1 Fahrenheit per year, this rate is twice that observed in the last thousand years. The average energy linked carbon dioxide release according to the Pew Center on Climate Change, have risen by over 130 percent since 1850; from two hundred million to 27 billion tons per year. This is projected to rise by a further 60 percent by 2030 (Afzal, p. 1). The Arctic and Antarctic ice caps are shrinking, the ice on mountain tops is melting, and the permafrost at the arctic is thawing. Scientific uncertainty exists concerning the severity and speed of global warming and climate modifications. Global warming is occurring for sure, and the health impact of this phenomenon will certainly necessitate informed responses by healthcare professions (Gray, p. 6-10). Overview of Climate Change and Global Warming Gre enhouse effects influence global warming. Greenhouse effects describe the rise in earth’s temperature due to the presence of such gases as methane, and carbon dioxide in the earth’s atmosphere. ... Human activity increases greenhouse gases production increasing their effects on earth. Greenhouse gases increase leads to lowered irradiation of infrared rays back to space. As a result, earth’s average temperatures rise, contributing to climate change (Afzal, p. 1). Hypothesis Climate change is a phenomenon that the world is currently experiencing. This is attested by climate changes worldwide. There has also been an increase of health complications related to climate change such as lung complications, skin and heart complications. This paper gives a brief overview of global warming and its effects on public health. Methods Statistical methods were used to analyze the problem. Earth’s temperature data collected from satellites in outer space, balloons and remote sites were compared to proxy temperature. Data from areas hard hit by climate change and global warming was used to study the health effects of the phenomenon on humans (Reiter, p. 5-14). Results Mounting evid ence suggests that global warming affects human health through events such as changes in water and air quality, extreme weather events as well as infectious disease ecology changes. Europe in 2003 experienced the hottest summer in years with average temperatures exceeding the normal temperature by 3.5 degrees Celsius. Close to 22,000 people died due to this heat wave. Chicago residents in 1995 experienced heat waves reaching 106o F, which resulted to the death of hundreds. In 2005, United States experienced the impact of an extreme weather event; Hurricane Katrina, which resulted to the death of 1,464 people. These events demonstrate the challenge of coming up with an effective and efficient public health response (Afzal, p. 1). During most of the flooding

Naccobus Essay Example | Topics and Well Written Essays - 2000 words

Naccobus - Essay Example The sources will be picked from the list that has been used in the provided study. Each source will be analyzed separately and the reason why it was important to use it will also be given and how it helped in making the paper complete. Sher is this is one of the most important sources that have been used in this study. This source has been used throughout the study to provide concrete information on Nacobbus. Sher gives the required information about the Genus Nacobbus (222-227). According to this source the genus contains only two valid species which are indicated to be the Nacobbus dorsalis and N. aberrans. This source goes ahead to state where these two species are normally found. It indicates that N. aberrans is normally an important parasite of various vegetables like beans, chili, tomatoes and pepper. Further into the study, Sher has been extensively used to give information on variability that has been seen in Nacobbus (222-227). The source has been used to give information on morphological characteristic of N. aberrans. It reveals the various ranges of morphometrics of N. aberrans from various geographical locations in the world, thereby helping in understanding the various characteristics of the organism being studied. The reason why Sher is considered one of the important sources that has been used is the amount of information that has been obtained from this source on the characteristics and morphology of N. aberrans. The second source that has been considered among the 10 most important sources in this study is Thorne (1949). Thorne is talking about the new classification of the Tylenchida. It is one the proceedings of the Helminthological Society of Washington. This source has been used to give information on various discoveries that were made on Nacobbus in different regions of the world. The source has been used to give information on taxonomy of Nacobbus (Thorne 57).

Personal statement Example | Topics and Well Written Essays - 500 words - 10

Personal Statement Example I have always had a goal of being a public administration officer (Stelzer, 243). My reason for applying for a master degree program in Public Policy and Management is that I deem that the course will help me in my prospect profession. I will be able to achieve my passion of being a Public Administration Officer. I always fancied public administration system and function, since my mentor in life was a public administrator situated in Saudi Arabia. This encouraged me to work and excel in my bachelor program, as I wanted to pursue a master course related to administration. I qualified in my Bachelor Degree in Mathematics highly. Thus, given an opportunity to study in California Lutheran University will be a communal relationship, since I am apprehensively ready to put into practice my readily acquired skills and experience. Furthermore, I am funded on my master degree program by the Saudi Arabian government. This will make my education unproblematic, since I will be prompt in paying my fees. My sponsor has guaranteed any necessary corporation regarding my education process with the university’s management. Though I am Saudi national, I have relocated to live in the USA, and I believe that this will be a rousing factor to enable me pursue my master program with zeal. I did not match the level of the American English, as a result, took an English course in the Cal Poly English language institute, in California, to match my skills with the standardized American language. While studying English at the institution, I was enthusiastic about studying the language and my vivid imagination accelerated the level with which I learnt English. I am now qualified in communicating and writing fluent English, and I believe will give me a humble timing in my studies, in the institution (Stelzer, 245). I am a fun enthusiast, gregarious and devoted person since I often enjoy jokes cracked by friends. The jokes

Marketing plan for a medical distribution company in Iraq

Marketing plan for a medical distribution company in Iraq This company believes that the success of its business is highly dependent on the strategies that the management will adopt in running the business. Accordingly, there was a need to establish a business model that would be used in operating the medical distribution office (scientific bureau). The company will implement the following two business models: Business model 1: Where the distribution office purchases products from the manufacturers, and then prices them before delivering them to customers. Business model 2: Where the distribution office acts as an agent on the manufacturers behalf, such that the manufacturer pays the distributor a commission on sales according to a mutual agreement. The scientific bureau will make use of both business models in order to maximize gains from the business. The first model will help the company to gain profits by selling quality products at affordable prices. This model is also flexible because it allows the business to set prices that are compatible to the situation at hand. An example is where the business may want to attract customers through lowering prices. The second model will be useful to the company because it will reduce financial pressure as well as bringing in more capital. The business will only be required to supply products to customers on behalf of manufacturers to get commission. This essentially means that no initial capital outlays will be required since the manufacturer will be liable for all expenses. This model appeals to multi-national companies who want to get a foothold in the developing economy of Iraq, since they cant come in themselves because of the volatile security situation. CORE STRATEGY Mission statement: To continuously facilitate clients by effective provision of medical supplies and services, while considering the social, political and religious beliefs of the surrounding environment. Market scope The market segmentation for the company will be based on faith communities. Baghdad, like many other provinces in Iraq, is divided into mainly Sunni and Shiia communities.  [1]  These are the two denominations of the Muslim faith. The companys employees will be highly trained and have knowledge of how to interact appropriately with each of these communities since their traditions, political beliefs and some religious beliefs are different. The distribution office will focus on these communities in Baghdad and beyond and will address the medication needs of hospitals, health centres, physicians, and pharmacies. The individuals category will mostly be served by the hospitals and pharmacies and will further be categorized into men, women and children. Notably, each of these customer focus groups has different needs, despite requiring common medications in certain circumstances. During the start up phase the company wont be targeting the Kurdish community that lives in the north of Iraq. This is because the company is unfamiliar with their language, traditions and the way they conduct business. However, during the growth stage the company may consider working as an indirect distributor to this community by building links with Kurdish traders. They in turn will target the Kurdish community. Because the security situation in Iraq continues to be volatile  [2]  , people continue to be injured on a daily basis. The company will provide the hospitals and pharmacies with the appropriate equipment, supplies and medication to deal with these injuries. In addition to that, the company will provide medical equipment needed to revive the health industry. This industry has been damaged by 30 years that saw the Iraq/Iran war and the 1st and 2nd Gulf Wars. As the business continues to grow, it is highly probable that the products portfolio is likely to expand such that more goods will be incorporated. Examples will include dental equipments, laboratory equipments such as microscopes, X-ray machines and other radiography machines. Basis for differentiation The distribution office will ensure that the goods obtained are of high quality and that counterfeit drugs like the ones possessed by other sellers are avoided. The pricing would be such that the distribution office makes enough profits but offers a price that is lower than that of competitors. This will most definitely attract customers to the office as they seek lower prices. This is an important strategy even for a start-up company because the economy in Iraq is developing slowly. The distribution office will be the first to introduce a loyalty program, which will reward regular customers with discounts. This loyalty program will be designed in such a way that customers gain points whenever they shop at the medical distribution office, which can then be redeemed in the form of products or reduction in prices. A certain number of points will allow customers to gain goods worth a specific amount of money. This, in turn will promote customer loyalty and consequently enhance the distribution offices differentiation. STRATEGIC RESOURCES Core competencies: The companys internal resources will give it a competitive advantage; mainly its knowledge capital and the dedication of the employees. These employees will have a high standard of knowledge about the companys products and after sales care. They will undergo training courses in all these elements and be required to up-skill on a regular basis in order to be promoted within the company. They will also be highly trained in communication and will have a great cultural knowledge, which will enable them to develop unique relationships with customers from different backgrounds. This approach is unique and will be essential for the success of this business. Furthermore, the companys strategy will embrace a combination of a wholesaler/agent model. This will ensure that the companys financial resources are not depleted; while the company continues to make profit. Also, the office will enhance customer needs satisfaction due to the availability of products and fair prices. The two business models will allow flexibility in such a way that the company can easily adjust to new opportunities. These could include the sale of medical equipment such as wheel chairs, radiography equipments and surgery kits among others. Strategic assets Apart from the medical supplies, the distribution office will have three transport vehicles that will be used to ferry products from the store to the customers location. These transport vehicles, will be driven by skilled drivers, who know the ins and outs of the city of Baghdad. This is essential due to the traffic disruptions caused by highly security measures and checkpoints.  [3]  This is to ensure that goods are delivered on time thus improving customer satisfaction. The distribution office will also have special forklifts that will be used in transferring items from the store to the loading zone during a sale and from the loading zone to the store during delivery. These forklifts will not only save time but also reduce the number of staff required to work at the store. These will set the business apart from competitors because we believe this strategic asset will give the businesses an added advantage over competitors. PARTNERSHIP NETWORK Supply chain management The distribution office will use an integrated supply chain that aims at connecting the manufacturers, retailers and customers. An integrated supply chain will help to join the functions of the firms involved so that they can easily connect to one another and obtain important information. The company believes that a supply chain can only perform effectively if the right technological infrastructure is utilised. This approach is new to the Iraqi market, which has not traditionally relied on technology. The medical distribution office will make use of supplier portals  [4]  . This technology has an advantage in that it combines a variety of activities including order management, demand visibility, bar code generation, advance ship notice functions and the generation of shipment requirements. This system will assist the medical distribution office in organising its supply chain in such a way that information flows effectively between the office, the retailers and the manufacturers. As a result, supply will be continuous and factors within the supply chain including demand and order replenishment will be addressed with ease. Other key relationships The business needs to establish proper relationships with other stakeholders other than the manufacturers and customers in order to enhance co-existence. Good business in Iraq is determined by how well connected the business owners are. The strength of relationships that they have with others in the business world, government representatives and within the community cannot be underestimated. The business will join the scientific bureau consortium so as to act together in dealing with issues such as government regulation. In essence, it is advisable for a business to be part of a group with similar interest. CUSTOMER INTERFACE a- Target market: As stated above, the medical distribution office will focus on Sunni and Shiia faith communities. This market segmentation plays an important role in ensuring that the needs of each segment are met. The company will focus on pharmacies, hospitals, and governmental bodies in these communities. It will then further divide the communities in terms of men, women and children. This allows for easy collection of information regarding the needs of a particular group so that they can be served more effectively. In essence, segmentation allows specialised treatment of clients. According to recent information on the World Health Organisation website  [5]  , the following are the most common illnesses that the company will be providing medication and services for: Water-borne diseases, such as typhoid fever and hepatitis A, are on the rise as a result of the damage to water and sewage systems caused by the war. Heart diseases are a leading cause of death both cardiovascular and rheumatic heart diseases are on the rise. The second one especially, because of the uncertainty of the security situation and the country experiencing three wars in the last 30 years. Upper respiratory tract and diarroheal infections are high among children. Incidences of childhood cancer are ten times that of developed countries. This is highly contributed to the emissions from bombs during the wars. There are inadequate supplies of chemotherapeutic drugs, which is an area the company can build on. Maternal mortality rates are very high as over 40% of births occur outside of health institutions. Upon entry into the Iraqi market, competitors will react negatively and may even attempt to push the distribution office out of business. The distribution office will need to be well prepared; which will involve a proper analysis between targets and the firms resource configuration. The company will be using the combination of its core competencies (as detailed above) and its supply chain technology to achieve its targets. This approach will minimise the threats of competitors and other new entries to the market. Once the company has been established, new opportunities such as increased business within the same industry or in a different setting may be undertaken thus increasing the business profitability. b- Fulfilment and support The business will make use of direct channels to distribute the products. From the manufacturer, the distribution office will sell or deliver them straight to the individuals or organisations buying the products. This direct approach will minimise the cost and enable the office to build good relations with both the manufactures and the clients. The client base will be served by a dedicated customer support staff that will help in recording order specifications so as to ensure the right products are bought. The skilled drivers will ensure that they are delivered in time. With time, the distribution office could have a few subsidiaries within the region to supplement the main office. c Pricing structure: The pricing strategy will depend on the prices offered by the manufacturer. The strategy for the medical distribution office will be to provide a price that is slightly below that of competitors. While this may impact on profitability, it will attract more customers to the office. BUSINESS RESEARCH Opportunities in setting up a medical distribution office in Iraq It emerged that the warfare that has existed in the country for the last 30 years has put the country in disarray and not many businesses have been able to operate at an optimal magnitude. Currently, Iraqs 29,671,605  [6]  population is served by only 150 medical distribution centres. This can be attributed to the serious damages to infrastructure that occurred during the wars  [7]  . Most of the offices that exist are either small or medium sized, with a capital value of between $1million to $3million US  [8]  . This indicates that the populations demands are not being met, that the competition is not stiff, and a well-organised medical distribution office is bound to excel in the Iraq market. Local population preferences The buying habits of Iraqi people are to buy medicine from local pharmacies, as they often skip the doctors diagnosis. However, if they do get a local doctors prescription, they prefer to buy their medicine from their local pharmacies because there is a lack of medical supplies in the hospitals  [9]  . For example, a Sunni person will visit their local Sunni doctor and buy their medication from a Sunni pharmacy. These findings support the approach that this medical distribution office is taking by targeting communities. At the start the Sunni and Shiia community and, at a later stage, the Kurdish community. While some Iraqi people preferred local products because their sources could be trusted, others were of the view that imported medicine is likely to be more effective especially if it comes from the developed countries where manufacturers use more advanced production methods. Statistically, 8 out of 10 of the participants  [10]  preferred to use imported medication while the others indicated they would rather use local medications. The group in support of the imported medication maintained that international companies tend to manufacture good quality products as opposed to local companies. They tended to trust imported medications more because they used them successfully before. Furthermore, they believed that as the industrial infrastructure is damaged in Iraq it is hard for these local factories to produce good quality products. The number of products they can produce also limits these local factories. As a result, they were afraid of trying out the local medications. Participants who preferred local medications did so because they had been using them for years and didnt want to switch. These findings suggest that the business should continually supply imported medications, as these are likely to attract higher demand than local medicines. (See figu re 1) 1-MACRO ENVIRONMENT ANALYSIS Political factors Political stability in Iraq is quite uncertain and businesses investing need to take proactive measures aimed at protecting their businesses from harm in case of an incident. It is notable that while things have cooled down and reconstruction efforts are underway, it may be hard to predict political unrest that is likely to occur. The political system in Iraq is composed of a number of parties of differing, specific faiths. They are either Sunni, Shiia or Kurdish parties. It is essential for the business to have political connections, as the level of corruption in Iraq  [11]  is so high that it would be very hard for a business to succeed without both political and financial support. Economic factors The International Monetary Fund predicted that Iraqs economy would grow by 7.9% in 2011  [12]  . This growth is linked to the improving security situation in the country. The level of employment will rise, therefore increasing the populations disposable income. Baghdad remains the most economically important city in Iraq, with the highest population. It is also regarded as the richest city in the country.  [13]  It is the capital city and is therefore bound to be the centre of all activity. Given its estimated population of 8 million people, the medical distribution office is likely to perform well in Baghdad. With continuous restructuring of the city to clear the effects of the war, normal business is expected to resume such that the business will perform well. Social-cultural factors Iraq is rich in terms of social and cultural aspects. Being a Muslim dominated country, with different denominations within that faith, it is notable that there are visible differences with the rest of the world in terms of social behaviour and business expectations. It is therefore important for this business to train its employees how to interact and communicate with these different communities. There are, however, some similarities that should be respected and complied with. Crossing ones hands or legs during a conversation, for example, is considered rude and should therefore be avoided. A handshake at the beginning and departure is highly regarded. Friendships and connections are highly emphasised in Iraq and it is common to find people doing business only with the people they know. Bribes are also common such that getting various tenders and privileges may require a business to part with a significant amount of money as a bribe unless the owners are well connected politically.à ‚  [14]   Technological factors Traditionally, most businesses in Iraq have not relied heavily on technology, beyond the basic use of emails, fax etc. One of the main obstacles for the use of technology has been the shortage of electricity. It is, however, an area that is developing rapidly in recent years. It is expected that it will be essential for the success of the business. This supports the companys plan to introduce supplier portals as part of its supply chain. Environmental factors Baghdad is centrally located and is therefore a viable location for business. Being at the centre of most economic activity in the country, Iraqs capital Baghdad is expected to have good returns for businesses. Since the security situation is still volatile, and the government is focusing on stabilising it and on the reconstruction of the damaged infrastructure, it is putting all its efforts into these areas. The implementation of environmental legislation is not a priority for the next few years. Legal and regulatory The legal system in Iraq is improving slowly. It is however notable that bias still exists within the system for people with money or political connections. Bribery is highly apparent and one is likely to be served faster if they give a bribe than when they do not. For the medical distribution office the regulation is that all the companys products must pass the quality control insurance department test in the Ministry of Health. Also, the company must register with KIMADIA  [15]  , which is a department in the Ministry of Health. This is the only authorised governmental body to which the company can tender in order to supply the governments needs for pharmaceutical products and equipment. If the company fails to comply with these policies, it will not be able to practice in the Iraqi market. Industry Analysis Prior to the costly Iraq/Iran war, the health industry in Iraq was characterised as being one of the best in the region. Following the first Gulf War in 1991, the UN imposed sanctions to encourage Iraq to disarm its weapons of mass destruction. The effect of this had disastrous effect on the economy, especially on the health sector. UNICEF estimated that 500,000 died as a result of a lack of medical supplies, clean water and malnutrition during this time. This sanction was lifted after the invasion of Iraq in 2003  [16]  . The industry remains unstable as most businesses are still recovering from the effects of the wars. As the economy in Iraq is emerging gradually the performance of the health industry is quite encouraging. It is estimated that the health infrastructure will need around $8 billion US to be revived. As Iraq is a country with 90% of its revenue coming from the sale of oil, therefore the development of the health sector largely depends on how many barrels of oil are exported. The Iraqi budget for 2011 will be approximately $86billion  [17]  , with around 7% (or approx. $6 billion) of it to be allocated to the health sector. This excludes the private sector health facilities (pharmacies, hospitals etc). This would indicate that there is room for more than the 150 medical small to medium size distribution offices that currently serves the population. Most of the private health care facilities, including the medical distribution offices, are locally owned. As a result, the population is more likely to identify with the owners, given similarity in culture. There are very few foreign owned businesses in Iraq because of the unstable security situation and the governments inability to protect foreign investors. (See figure 1) (Figure 1) Ranking of Iraq in Protecting Investors Compared to good practice and selected economies  [18]   The health sector in Iraq is controlled by government regulation. It dictates the types of medication and medical equipment that can be brought into the country. However, the government has no legislation as regards the price of products. This falls under the individual medical distribution offices remit. Competitor Research: There are a number of scientific bureau (medical distribution offices) competitors in Baghdad. Most of these businesses are small to medium sized businesses, all of which aim at providing medication and medical equipment. The major and most respected competitors are Al Assad Scientific Bureau, Al Bashaer Scientific Bureau, and Kasim Irzzouki Scientific Bureau. Al Assad is a leading distributor and has been a main vendor for the Ministry of Health. It is well known and enjoys a high market share both in Baghdad. However, the company does not have any branches or market share in the Shiia southern region of Iraq, especially Basra. In addition to that the company has not won any tenders with the Ministry of Health for the last two years. Kasim Irzzouki is the main distributor in Iraq for Organon.  [19]  This company mainly produces anti-impotence drugs for both men and women. It has the major market share in this area and distributes all over Iraq. Al Bashaer has a wide range of Egy ptian and Indian medical products. They are well known for supplying hospitals with disposable items such as cannulae and Foley catheters. Neither Al Assad or Al Bashaer has done well outside of Baghdad. It is worth noting that none of the major competitors supply chemotherapeutic drugs, even though there is a need in the Iraqi market due to the high incidences of childhood cancer. Market analysis, planning and research The population of Baghdad is approximately 8 million. This is a considerable market size and the business could benefit immensely if it could manage to get a large market share. During the start-up phase the companys operations will be conducted in Baghdad. There are approximately 10,000 physicians in Baghdad  [20]  . Furthermore, there are around 1,500 pharmacies in the city  [21]  . There are three main governmental hospitals and three main private sector hospitals. This indicates a large potential client base for that the medical distribution office. It is notable that the medication market has widened significantly. It is projected that in the next few years, as the security situation and the economy in Iraq improves there will be a return of pharmacists and doctors who fled, for their own safety, during the last 20 years. There will also be an increase in the number of hospitals, and it is projected that the population will increase. Baghdad is divided into two main regions, Al Kahr and Al Risafa. Each of these regions has both Sunni and Shiia communities living in separate areas. There are only a few areas where you will find a mix of these communities living together. There are other minority groups too, but the company will not focus on them at first. The market strategy for the company is to target the hospitals, clinics, pharmacies and medical centres in each of these areas. The company will rank these potential clients in terms of the number of people they treat on a daily basis. During the start-up phase the priority will be to target those with the largest client base and build a relationship with them. The company will also target those with a smaller client base, but with less frequency. The Ministry of Health will be one of the major potential clients. In the expansion phase, the company will target the other provinces in southern and northern Baghdad. The southern provinces of Baghdad are mainly Shiia communities, while the provinces north of the city, as far up a Kurdustan, are Sunni communities. As previously mentioned, the knowledge capital of the companys employees will play an important role in its development. The staff will have a wealth of cultural knowledge, which is essential for the business. They will be highly skilled, which will enable them to build and maintain good relationships with customers from different communities. Repeat business should occur once good relations have been established between the institution or individual and the medical distribution office. The company will also adopt Internet advertising. The web site will include the companys details such as the address and the products they deal in, with the objective of informing customers about their work. Most of the Internet posts will contain the businesses achievements and well-known clients in order to promote a professional look. In addition to raising more capital, the companys growth strategy is to implement the same ideology in provinces other than Baghdad. It may also branch into sales in the Kurdish community as an indirect supplier, and introduce new products to the Iraqi market. ORGANISATION MANAGEMENT AND OPERATIONS Management team and model The team The management team will consist of the board of directors, the Chief Executive Officer, the human resource manager and the head of accounts. This team will be responsible for steering the business through addressing various aspects. The CEO will oversee the operations of the company and manage resources in such a way that the business objectives are met. The CEO will govern every employee in the company through the Human Resource Manager. The business will employ two HR officials, one of who will be the HR manager. He or she will oversee the business human resource needs and ensure that the business is well equipped with people skills to enhance smooth operations. The HR will govern the hiring process, training, payment issues and employee welfare within the business. One of the employees in the accounting department will act as the head of accounts. He or she will ensure that financial resources within the business are well managed and accounted for so as to ensure that the needs o f the business are met effectively. The head of accounts will also be responsible for effectual distribution of resources within the firm. Management model The management will apply the management by objectives model (MBO). Under this method, the company defines objectives, which should be met by all employees and then compares their performance against these objectives. This model is effective in maximizing performance and avoids indulgence in activities that deviate employees from their objectives. Accordingly, the business is likely to succeed as opposed to where employees efficiency is unaccounted for. Management philosophy Concern for employees The business intends to maintain an integrated system, whereby the management will oversee efficiency in the firm while allowing employees to actively participate in the management process. The employees remain the major assets of the firm and are definitely responsible for the success of the business. The business will go to every length to ensure that it finds; hires and trains good employees and treat them like family. The management will ensure that they communicate effectively with employees and continually acknowledge their importance through proper reward systems and involving them in management decision making. Hands-on management Managers will be expected to know the business comprehensively and guide employees through the accomplishment of the business main objectives. They will continually interact with customers and employees in order to effectively understand and meet their needs. Managers will set the pace for the employees and as many business people would attest, employees are likely to wo

Theme of Hypocrisy in Conrad’s, Heart of Darkness, and Coppola’s, Apocalypse Now :: Literary Comparison, Analytical Essays

Both Conrad’s, â€Å"Heart of Darkness†, and Coppola’s, â€Å"Apocalypse Now†, profoundly illustrate the journey of man into their inner self and man’s encounters with their insanity, fears and demise. The novella and film are comprised of numerous pivotal themes that facilitate the understanding of the deeper meaning of both works. Fundamentally, theme is an extensive message or idea expressed by an author and is a crucial element of literature since it sheds light on universal concepts. The most striking parallels that can be formulated when comparing themes in both the novella and the film are associated with human nature. Specifically, Conrad and Coppola incorporate theme of hypocrisy in order to portray man’s incredible potential for evil. Firstly, the theme of hypocrisy is integrated in both works for the purpose of portraying man’s staggering and absurd potential for evil. In the novel, Heart of Darkness, the Europeans state that their objectives in Africa are to trade with the natives and immerse them with the light of civilization. However, their actions fail to reflect their stated motives since the Europeans take the ivory from the natives by force and they treat them inhumanely. Not to mention that the Europeans constantly refer to natives as objects such as machinery as well as suppress and eradicate them at any opportunity. Ultimately, the Europeans utilize their false words as a civilized veneer that masks their capability of being evil and savage. For instance, in Africa, Marlow states that he has familiarized himself â€Å"†¦with a flabby, pretending, weak-eyed devil of a rapacious and pitiless folly.† (pg. 81). Symbolically, the colonists are described as the devil mentioned above becau se they treat the natives with brutality even though their stated intentions are pure. Also, the colonists are foolish and tactless for they are unaware that their actions expose the evilness behind their actions. Similarly, in Coppola’s work, the United Sates affirms that they fight in the name of freedom and democracy, yet they commit senseless and violent killings. In reality, their ulterior motives were to promote capitalism and become a dominant military world power. Furthermore, the U.S. focuses on their attention on murdering their own operative instead of attempting to finish the war, which is of a higher priority when lives are at stake. Additionally, the U.S. troops are strangers in Vietnam, but they act as if posses the foreign land since they seize territory and commit murder without being incited.

Sexual Passion in The Turn of the Screw :: Turn of the Screw Essays

Sexual Passion  in  The Turn of the Screw   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   In a criticism on Henry James’s story The Turn of the Screw, Strother Purdy suggests that large amounts of sexual passion may be assumed to exist underneath the surface of the narrative. Purdy says that under a Freudian interpretation of the story, the sexual element is easily recognized and is used as the whole source of the action. According to this theory, the governess wishes to impress her master because she is in love with him and, therefore, exceeeding her authority with the children. Although the governess only sees her master twice, Purdy refers back to what Douglas had said,† it was the beauty of her passion.† Since the master is not impressed by her initial and ordinary course of governessing, she must make up some life-threatening danger to the children so she can rescue them and win the masters love and affection. She figures the danger must be terrible because he told her he did not want to be bothered with matters dealing with the children. He basica lly tells her he cares nothing for the children. Purdy suggests the governess is unconscious in doing this because she is sexually repressed and cannot admit her sexual motives to herself She believes she is actually protecting the children against an outside evil, which happens to coincide with her drive to demonstrate heroism and devotion to the master. According to Purdy, the governess conjures up ghosts because she invited them and willed them to come. Purdy feels the housekeeper plays her trump card and the governess' concern is not for the children but for all that would become of her, her bargain with the master, her relationship, and her passion for him. Purdy finds the following quotes to show her passion for the master and show that this serves as the motive of action. â€Å"She did not know- no one knew- how proud I had been to serve him and to stick to our terms.† â€Å"I set in motion the fine machinery to attract his attention to my slighted charms.

Group Manuscript

Bacterial cells are a common choice for in vivo replication of DNA of interest, and in this study, the heat shock method was employed for bacterial transformation. Plasmids, which are DNA molecules themselves, were used as expression vectors for the DNA of interest, the GAP gene. Because only transformed cells exhibit antibiotic resistance, trans armed cells survived on plates containing inclining. Only those cells that took up plasmid s containing the GAP gene fluoresced in IV light.By restriction enzyme analysis and gel electro prophesiers, the relationship between genotype and phenotype was observed using isolated p zamias from the bacteria. Because the presence of the GAP gene codes for fluorescence, it is expected t hat a genotype coding for the protein would express the glowing phenotype. Regular action of GAP gene expression was observed in samples that were grown with rabbinate, inimical in, and varying amounts Of glucose. Our results Suggest that the presence Of glucose in t he s rounding environment inhibited transcription from the rabbinate bad promoter. Age 3 INTRODUCTION This study examined the transcriptional regulation Of the rabbinate Oberon pr emoted found in Escherichia coli (E. Coli). To facilitate this study, the Green Fluorescence t protein (GAP) was utilized as a reporter gene with the rabbinate promoter. The KEEP gene w as 772 base pairs (BP) long and was extracted from Quarrel Victoria. This gene was implanted into plasmids, which were inserted into the E. Coli through bacterial transformation (3). In order to obtain enough copies of these DNA samples in a reasonable ammo NT of time, two methods can be used.The first is considered in vitro , or in glass, (such as a test tube) and is known as polymerase chain reaction. This method, in which a machine heats t he DNA sample ND Tag polymerase clones the DNA, is expensive and less convenient, so it is not always used. The other method is In Vivo , or in life, and is called transformation. This method was used in this experiment by shocking E. Coli bacteria with heat in order for them to take in plasmids that were transformed to contain the GAP gene. Not all bacteria were transformed, and not all transformed bacteria contained the GAP gene.To differentiate between transformed and n untransformed bacteria, they were grown on inclining, as the transformed bacteria were rest assistant to impact Olin but the untransformed bacteria were killed by it. The plasmids with and with out the GAP gene were differentiated by visualization under IV light (4). The genotype of the remaining plasmids, both transformed and nontransparent med, were then tested to determine the relationship between the genotype, or plasmid c imposition, and phenotype, or presence of fluorescence.The plasmids were removed from the e bacteria, with some samples left whole as controls and others cut into pieces by restriction enzymes. In this case, doll, originating from Hemophilia influenza , was the enzyme use d to cut the plasmids Page 4 at their respective Hind doll sites, where the GAP gene would have been inserted. This was done to determine whether or not the KEEP gene was taken in by the plasmids when it was electrophoresis, as the difference in size of the pieces was observed in the gag arose gel (4).Even though a transformed bacterium may have had the GAP gene in its insert Ted plasmid, it needed the promoter bad (consisting of genes Arab, area, and award) and t he GAP gene in the right direction and position in order for the bacterium to have the potent al for fluorescence. It may still not have fluoresced if there was not enough rabbinate present for the bad promoter to run, or if a high concentration of glucose inside the cell was present to rep as the bad promoter.With all the necessary genetic coding for fluorescence, the amount that was visible depended on the amount of glucose present, as no glucose caused it to floorer see brightly, and a low concentration made it glow dul ly (4). Each step of this experiment was vital in analyzing the transcriptional regulate on of the rabbinate Oberon promoter. Through bacterial transformation, recombinant DNA and cloning methods were used in order to insert the GAP gene into the plasmid.The pellet of cells was then resume need using a pipette and vortex mixer. This allows for a XX concentration to be obtained. After spreading, the four plates were incubated upside down (overnight at 370 C) in a microbial incubator. Following incubation each plate was placed upside down n a IV box and photographed. Restriction Analysis In restriction analysis, two restriction enzyme digestion reactions (one uncut b Y enzyme, one cut by enzyme) occurred for each of the two DNA samples (nonresistant and non page 6 fluorescent, resistant and inflorescent, and resistant and fluorescent).For the uncut samples, components were added in the following order: Pl XIX Buffer ( supplied by environment by life technologies†Ã¢â‚¬Ëœ containi ng: 100 mm Trisect, pH 7. 5 100 mm Magical mm Theoretical 500 mm Nasal), 1 Pl Water, and 5 Pl DNA For the uncut samples, components were e added in the allowing order: Pl XIX Buffer, Pl Water, pi DNA, and III Handbill NZ. (sup plied by invitation by life technologies†Ã¢â‚¬ ). After all components were added they were mixed by overexerting and collected at the bottom of the tubes by using the microelectronic gem The tubes were then incubated for 30 minutes at 37 co.Following incubation, Pl of XIX loading g buffer (1% (w/v) SD (sodium decoded sulfate) 50% (v/v) glycerol 0. 05% (w/ v) bronchiole blue) was added to each tube. The samples were mixed by overexerting and collected at the e bottom of the tubes using the microelectronic- Agrees gel electrophoresis was prepared by ding Pl of 1 KGB plus ladder ( supplied by environment by life technologies†Ã¢â‚¬ ) into the first and final well. 12. Pl of each sample was then loaded into remaining wells on the gel and the electro prioress was run for 1 hour at 1 VIVO.The gels were then placed in a IV box and photographed. Using the photo of the gel, the genotypes of each sample were verified. This was done by finding the size of fragments through comparison with the DNA standard ladder. Gene Expression TO allow for observation Of phenotypes gene expression, bacteria were transfer erred to three types of media: inducing, introducing, and repressing. Five plates were used: LB,'Amp, LB/Marry, LB/Amp/AR/Glue 0. 2%, LB/Amp/AR/Glue 0. 5%, LB/ Amp/AR/Glue 2% (xx).On each plate, bacteria with empty plasmids and therefore no GAP gene for flour essence were spread on one half, and bacteria with plasmids containing the GAP insert were e transferred to the Page 7 other. Both of these types of cells came from a master plate. Using a sterile to toothpick, each type of bacteria was patched in the appropriate area of each plate. GAP+ bacteria were patched in a â€Å"+† shape, while GAP bacteria were patched in a shap e. Plates were labeled properly and Leary and were placed Poseidon in a ICC incubator overnight.Plates were e observed for fluorescent bacteria on the IV transformational box after 24 hours, after 72 h ours, and again after 96 hours. Page 8 RESULTS Bacterial Transformation In order to investigate GAP gene expression, it was first necessary to obtain co pies of the DNA of interest through bacterial transformation, which allowed for plasmid ( and sometimes GAP) uptake by numerous bacterium. Phenotypes results from this procedure can suggest possible genotypes. Transformation plates that were prepared after heat shoo KC transformation can be Seen in Figure 2.Individual colonies were visible on each AMP+ plate, while a lawn of bacteria had formed on the AMP plate and individual colonies were not discern enable. Under IV light, fluorescence was observed in those colonies expressing the GAP gene. If guru E highlights the difference observed between glowing and knowing bacterial colonies. The fraction of colonies that appear fluorescent and are assumed to be GAP+ is noted in Table e 1 along with complete results of this bacterial transformation (4).Structural Analysis by Restriction Analysis and Gel Electrophoresis TO confirm the structure Of DNA plasmid genotypes, samples were run through h gel electrophoresis after being treated with a restriction enzyme specific for cleave ins the gene of interest (GAP). Standards were run along with each of the components on the electrophoresis gel. In order to determine size in base pairs of fragments of interest, a graph of the e relationship between the size and migration of the bands in the 1 KGB plus DNA Ladder was assembled (Figure 4), and a line of best fit was determined.The relationship between the base 1 O log of size and migration is linear, and graphing them together gave a trend line with an ex. action useful in determining the size of experimental fragments with known migration values. These were the fragments obtai ned by cleaving the plasmids with the Handbill restriction enzyme. Table 2 page 9 organizes the sizes and migration distances for the fragments of the standard included during electrophoresis. These values were used to construct the calibration curve m mentioned before (Figure 4).Figure 3 shows the agrees gel obtained by gel electrophoresis. Ta able 3 lists all sizes determined based on comparison with the calibration curve generated from migration standards (Figure 4). Sizes are noted for both the vector and the insert (4). Analysis of Reporter Gene Expression Investigation of gene regulation and interaction of environmental rabbinate a ND/or glucose with genotype required GAP+ cells to be spread on various plates, an d fluorescence to be observed over time.Table 4 summarizes the observations of the phenotype o f patches streaked onto AMP plates containing or lacking rabbinate and/ or glucose. Glowing patches suggest expression of the GAP gene. Observations show that the rabbinate sample pop site for the GAP insert fluoresced brightly as time went on. The sample with rabbinate and 0. 2 % glucose increasingly fluoresced over time, while plates higher in percentage glucose c imposition did not fluoresce. Cells that were GAP were also spread in order to serve as a surrogate et marker.These cells do not contain the gene for GAP, so they will not fluoresce under IV light . This gives a comparison, making it easier to determine if cells are expressing GAP fluoresce once or not page 10 DISCUSSION Regulation of Gene Expression: The samples that were grown with rabbinate, inclining, and varying amounts of glucose showed that the presence of glucose in a bacterium's surrounding environment NT can affect the ability of its rabbinate bad promoter. The plate with no glucose added flour cede brightly, while the plates with glucose added showed very little to no fluorescence.The only plate with glucose added that fluoresced in the end was the plate with the least glucose ad ded These results are due to the glucose inhibiting the rabbinate Oberon from trap inscribing the bad promoter DNA. When glucose is present in a bacterium, the cell metal likes the glucose instead of the rabbinate, and the rabbinate Oberon is not utilized. However, when the cell is lacking glucose, it reaches a state of â€Å"hunger† and begins producing cyclic adenosine Mephistopheles (CAMP). This reacts with the CAMP receptor protein (CROP), who chi allows the cell to use rabbinate to induce the transcription of the rabbinate bad prom otter.This promoter contains the genes Arab area , and award, which are part of the rabbinate Oberon. This system can only function if rabbinate is present in the cell; otherwise the gene arc will prevent the rabbinate Oberon from carrying out transcription by forming a â€Å"knot,† or loop in the DNA The rabbinate bad promoter reacts with the GAP gene to show when the Arabian SSE Oberon is in use and how strongly it is induced by rabbi nate (5). The plate with the least glucose added began to glow over time, as the cell be an using up the glucose in its environment by metabolize it for energy.It started to FL recurrences dully once the concentration of glucose was not high enough to fully repress the AR baboons Oberon, showing that there is a range of repression and induction, not just a state of † on† and â€Å"off' for page 11 these function. The more the bacteria used the glucose, the less of it was arrow ND to repress the Oberon, which is why its fluorescence strength grew over time. If the study WA s to be continued past the 96 hour mark, all of the plates would have eventually fluoresced as t hey used up their loses resources and began activating the rabbinate Oberon (4).